Parallel differential mobility analysis for electrostatic characterization and manipulation of nanoparticles and viruses

Autor(en)

Günter M Allmaier, Anne Maißer, Christian Laschober, Paul Messner, Wladyslaw Szymanski

Abstrakt

The electrophoretic mobility of charged, airborne nanoparticles (NPs) or macromolecules and their specific complexes opens new avenues for their analysis and handling. The newly developed parallel differential mobility analyzer in combination with an electrostatic particle sampler enables not only the characterization of bio-NPs, but even their sampling while preserving their bioactivity (e.g., the enzyme activity of galactosidase). Precondition for the applicability of this technique is a well-defined charging status of the NPs in question. This charge conditioning can be achieved by means of a radioactive source, Po-210, even if the yield in terms of charged particles is low for sub-20-nm particles and the aging of the source influences the size spectra measured. Nevertheless, this technique enables size-defined sampling and enrichment, combined with real-time measurement of the size of both NPs and viruses. Furthermore, it allows determination of the number of attached biospecific antibodies, thereby providing information about the surface coverage of viruses by antibodies.

Organisation(en)

Aerosolphysik und Umweltphysik

Externe Organisation(en)

Technische Universität Wien, Universität für Bodenkultur Wien

Journal

Trends in Analytical Chemistry

Band

30

Seiten

123-132

Anzahl der Seiten

10

ISSN

0165-9936

DOI

https://doi.org/10.1016/j.trac.2010.10.008

Publikationsdatum

2011

Peer-reviewed

Ja

ÖFOS 2012

103008 Experimentalphysik, 210006 Nanotechnologie

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/eb7278e2-fe6f-4dca-8826-32785f94210b