Laser-Induced Acoustic Desorption of Natural and Functionalized Biochromophores

Autor(en)

Ugur Sezer, Lisa Woerner, Johannes Horak, Lukas Felix, Jens Tüxen, Christoph Goetz, Alipasha Vaziri, Marcel Mayor, Markus Arndt

Abstrakt

Laser-induced acoustic desorption (LIAD) has recently been established as a tool for analytical chemistry. It is capable of launching intact, neutral, or low charged molecules into a high vacuum environment. This makes it ideally suited to mass spectrometry. LIAD can be used with fragile biomolecules and very massive compounds alike. Here, we apply LIAD time-of-flight mass spectrometry (TOF-MS) to the natural biochromophores chlorophyll, hemin, bilirubin, and biliverdin and to high mass fluoroalkyl-functionalized porphyrins. We characterize the variation in the molecular fragmentation patterns as a function of the desorption and the VUV postionization laser intensity. We find that LIAD can produce molecular beams an order of magnitude slower than matrix-assisted laser desorption (MALD), although this depends on the substrate material. Using titanium foils we observe a most probable velocity of 20 m/s for functionalized molecules with a mass m = 10 000 Da.

Organisation(en)

Quantenoptik, Quantennanophysik und Quanteninformation, Department für Strukturbiologie und Computational Biology

Externe Organisation(en)

Universität Basel, Karlsruher Institut für Technologie

Journal

Analytical Chemistry

Band

87

Seiten

5614-5619

Anzahl der Seiten

6

ISSN

0003-2700

DOI

https://doi.org/10.1021/acs.analchem.5b00601

Publikationsdatum

06-2015

Peer-reviewed

Ja

ÖFOS 2012

103016 Laserphysik, 104002 Analytische Chemie

Schlagwörter

ASJC Scopus Sachgebiete

Analytical Chemistry

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/e98ab944-df1e-4bb0-b875-58eb13068b1f