Optical refocusing three-dimensional wide-field fluorescence lifetime imaging microscopy

Autor(en)

Qiang Wu, Shangyu Guo, Yinxing Ma, Feng Gao, Chengliang Yang, Ming Yang, Xuanyi Yu, Xin-Zheng Zhang, Romano Rupp, Jing-Jun Xu

Abstrakt

Three-dimensional fluorescence lifetime microscopy is achieved by combining wide-field fluorescence lifetime imaging with a remote optical refocusing method. As required for some applications in dynamic research for physics, chemistry, or biology, it is thereby not necessary to move the sample, i.e., the specimen is not disturbed during measurement. Using a fluorescent microsphere the performance of the system has been tested successfully with respect to three-dimensional fluorescence lifetime microscopy as well as time-resolved fluorescence spectroscopy.

Organisation(en)

Physik Funktioneller Materialien

Externe Organisation(en)

Nankai University

Journal

Optics Express

Band

20

Seiten

960-965

Anzahl der Seiten

6

DOI

https://doi.org/10.1364/OE.20.000960

Publikationsdatum

2012

Peer-reviewed

Ja

ÖFOS 2012

103018 Materialphysik

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/e87325e3-ae2e-4dc0-a05d-6782902f0056