Strategies for the covalent conjugation of a bifunctional chelating agent to albumin: Synthesis and characterization of potential MRI contrast agents

Autor(en)

Amitava Kundu, Herwig Peterlik, Martin Krssak, Anna Bytzek, Irena Pashkunova-Martic, Vladimir Arion, Thomas H Helbich, Bernhard Keppler

Abstrakt

With the purpose to develop macromolecular magnetic resonance imaging contrast agents, we herein report three different synthetic approaches to the covalent attachment of bifunctional chelating agents to human serum albumin followed by coordination to contrast enhancing gadolinium(III). Applied methods cover active ester-mediated conjugation, linkage through glutaryl spacer, as well as the connection by the employment of glutaraldehyde. The content of gadolinium(III) was evaluated by inductively-coupled-plasma mass-spectrometry (ICP-MS) measurements and indicated reproducible amounts of conjugated contrast enhancing material. Small angle X-ray scattering (SAXS) experiments provided the size and altered shape of the gadolinium loaded proteins in comparison to unmodified albumin. Finally, the magnetic resonance properties of the protein conjugates were evaluated. The results indicated suitability of the gadolinium(III) loaded protein conjugates for use as macromolecular contrast agents in magnetic resonance imaging (MRI).

Organisation(en)

Institut für Anorganische Chemie, Dynamik Kondensierter Systeme

Externe Organisation(en)

Medizinische Universität Wien

Journal

Journal of Inorganic Biochemistry

Band

105

Seiten

250-255

Anzahl der Seiten

6

ISSN

0162-0134

DOI

https://doi.org/10.1016/j.jinorgbio.2010.10.015

Publikationsdatum

2011

Peer-reviewed

Ja

ÖFOS 2012

104003 Anorganische Chemie, 103018 Materialphysik, 301304 Medizinische Biologie

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/a917e756-fdf7-41a0-b47e-7e0bf68407f4