Real-time imaging of autofluorescence NAD(P)H in single human neutrophils

Autor(en)

Leiting Pan, Xin-Zheng Zhang, Kun Song, Bai-Quan Tang, Wei Cai, Xian Wu, Romano Rupp, Jing-Jun Xu

Abstrakt

Real-time detection of NAD(P)H is particularly important for understanding physiological activities of neutrophils. We scrutinize the performance of weak light detection systems with electron multiplying CCDs (EMCCDs) with regard to the feasibility of valid investigations by autofluorescence NAD(P)H in single human neutrophils. The low-noise amplification facility of EMCCDs is indeed just adequate to permit detection at an irradiation level where neither quenching nor phototoxic effects occur. For demonstration, a neutrophil respiratory burst was triggered and observed in real time. Our low-intensity detection system fulfills all requirements for real-time investigations at high spatiotemporal resolution in the field of neutrophil physiology and pathology.

Organisation(en)

Physik Funktioneller Materialien

Externe Organisation(en)

Nankai University

Journal

Applied Optics

Band

48

Seiten

1042-1046

ISSN

0003-6935

DOI

https://doi.org/10.1364/AO.48.001042

Publikationsdatum

2009

Peer-reviewed

Ja

ÖFOS 2012

103021 Optik

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/a217930e-c23b-440d-80d7-846a67eba253