amoA-based consensus phylogeny of ammonia-oxidizing archaea and deep sequencing of amoA genes from soils of four different geographic regions

Autor(en)

Michael Pester, Thomas Rattei, Alexander Gröngröft, Andreas Richter, Jörg Overmann, Barbara Reinhold-Hurek, Alexander Loy, Michael Wagner

Abstrakt

Ammonia-oxidizing archaea (AOA) play an important role in nitrification and many studies exploit their amoA genes as marker for their diversity and abundance. We present an archaeal amoA consensus phylogeny based on all publicly available sequences (status June 2010) and provide evidence for the diversification of AOA into four previously recognized clusters and one newly identified major cluster. These clusters, for which we suggest a new nomenclature, harboured 83 AOA species-level OTU (using an inferred species threshold of 85% amoA identity). 454 pyrosequencing of amoA amplicons from 16 soils sampled in Austria, Costa Rica, Greenland and Namibia revealed that only 2% of retrieved sequences had no database representative on the species-level and represented 30-37 additional species-level OTUs. With the exception of an acidic soil from which mostly amoA amplicons of the Nitrosotalea cluster were retrieved, all soils were dominated by amoA amplicons from the Nitrososphaera cluster (also called group I.1b), indicating that the previously reported AOA from the Nitrosopumilus cluster (also called group I.1a) are absent or represent minor populations in soils. AOA richness estimates on the species level ranged from 8-83 co-existing AOAs per soil. Presence/absence of amoA OTUs (97% identity level) correlated with geographic location, indicating that besides contemporary environmental conditions also dispersal limitation across different continents and/or historical environmental conditions might influence AOA biogeography in soils.

Organisation(en)

Externe Organisation(en)

Universität Hamburg, Leibniz-Institut DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen, Universität Bremen

Journal

Environmental Microbiology

Band

14

Seiten

525-539

Anzahl der Seiten

15

ISSN

1462-2912

DOI

https://doi.org/10.1111/j.1462-2920.2011.02666.x

Publikationsdatum

2012

Peer-reviewed

Ja

ÖFOS 2012

106022 Mikrobiologie

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/2882a6ad-a423-445e-85a2-a7cb74dbb389