Enhancing anti-adhesion properties by designing microstructure - the microscopy and spectroscopy study of the intercellular bacterial response

Autor(en)

Agnieszka Teresa Krawczynska, Anna Michalicha, Przemyslaw Suchecki, Karolina Budniak, Agata Roguska, Michael Kerber, Daria Setman, Maciej Spychalski, Boguslawa Adamczyk-Cieslak, Maciej Oskar Liedke, Maik Butterling, Eric Hirschmann, Andreas Wagner, Malgorzata Lewandowska, Anna Belcarz

Abstrakt

This study is the first one that investigates in detail the bacterial intercellular response to the high density of crystallographic defects including vacancies created in Cu by high pressure torsion. To this aim, samples were deformed by high pressure torsion and afterward, their antibacterial properties against Staphylococcus aureus were analyzed in adhesion tests. As a reference an annealed sample was applied. To avoid the influence of surface roughness, specially elaborated conditions for surface preparation were employed, which do not introduce defects and assure comparable surface roughness. The analysis of the chemical composition and thickness of passive layers by X-ray photoelectron spectroscopy showed that they were comparable for nanostructured and micrograined samples, consisting of Cu2O and CuO, and a thickness of 6 nm. The interface bacterium-substrate was prepared by a focused ion beam and further analyzed by scanning transmission electron microscopy and energy dispersive spectroscopy. High pressure torsion processed Cu shows enhanced anti-adhesion properties while in contact with S. aureus than micrograined Cu. There is a linear correlation between luminous intensity and grain size−0.5. The bacterial intercellular defence mechanism includes the creation of Cu2O nanoparticles and the increased concentration of sulphur-rich compounds near these nanoparticles.

Organisation(en)

Physik Nanostrukturierter Materialien

Externe Organisation(en)

Warsaw University of Technology, Medical University of Lublin, Polish Academy of Sciences (PAS), Helmholtz-Zentrum Dresden-Rossendorf

Journal

Scientific Reports

Band

14

Anzahl der Seiten

15

ISSN

2045-2322

DOI

https://doi.org/10.1038/s41598-024-75045-5

Publikationsdatum

10-2024

Peer-reviewed

Ja

ÖFOS 2012

106002 Biochemie, 104017 Physikalische Chemie, 210004 Nanomaterialien

ASJC Scopus Sachgebiete

General

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/71b3f5f6-26c2-4de1-b952-358a4f3ff52a