Ecophysiology of an uncultivated lineage of Aigarchaeota from an oxic, hot spring filamentous 'streamer' community

Autor(en)

Jacob P Beam, Zackary J Jay, Markus C Schmid, Douglas B Rusch, Margaret F Romine, Ryan de M Jennings, Mark A Kozubal, Susannah G Tringe, Michael Wagner, William P Inskeep

Abstrakt

The candidate archaeal phylum 'Aigarchaeota' contains microorganisms from terrestrial and subsurface geothermal ecosystems. The phylogeny and metabolic potential of Aigarchaeota has been deduced from several recent single-cell amplified genomes; however, a detailed description of their metabolic potential and in situ transcriptional activity is absent. Here, we report a comprehensive metatranscriptome-based reconstruction of the in situ metabolism of Aigarchaeota in an oxic, hot spring filamentous 'streamer' community. Fluorescence in situ hybridization showed that these newly discovered Aigarchaeota are filamentous, which is consistent with the presence and transcription of an actin-encoding gene. Aigarchaeota filaments are intricately associated with other community members, which include both bacteria (for example, filamentous Thermocrinis spp.) and archaea. Metabolic reconstruction of genomic and metatranscriptomic data suggests that this aigarchaeon is an aerobic, chemoorganoheterotroph with autotrophic potential. A heme copper oxidase complex was identified in the environmental genome assembly and highly transcribed in situ. Potential electron donors include acetate, fatty acids, amino acids, sugars and aromatic compounds, which may originate from extracellular polymeric substances produced by other microorganisms shown to exist in close proximity and/or autochthonous dissolved organic carbon (OC). Transcripts related to genes specific to each of these potential electron donors were identified, indicating that this aigarchaeon likely utilizes several OC substrates. Characterized members of this lineage cannot synthesize heme, and other cofactors and vitamins de novo, which suggests auxotrophy. We propose the name Candidatus 'Calditenuis aerorheumensis' for this aigarchaeon, which describes its filamentous morphology and its primary electron acceptor, oxygen.The ISME Journal advance online publication, 3 July 2015; doi:10.1038/ismej.2015.83.

Organisation(en)

Externe Organisation(en)

United States Department of Energy, Montana State University, Bozeman, Indiana University Bloomington, Pacific Northwest National Laboratory, Sustainable Bioproducts LLC

Journal

The ISME Journal: multidisciplinary journal of microbial ecology

Band

10

Seiten

210–224

Anzahl der Seiten

15

ISSN

1751-7362

DOI

https://doi.org/10.1038/ismej.2015.83

Publikationsdatum

01-2016

Peer-reviewed

Ja

ÖFOS 2012

106022 Mikrobiologie

ASJC Scopus Sachgebiete

Ecology, Evolution, Behavior and Systematics, Microbiology

Sustainable Development Goals

SDG 15 – Leben an Land

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/0b1231dd-9590-47ed-bc7e-74dc21c98cab