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Ecophysiology of an uncultivated lineage of Aigarchaeota from an oxic, hot spring filamentous 'streamer' community

Autor(en)
Jacob P Beam, Zackary J Jay, Markus C Schmid, Douglas B Rusch, Margaret F Romine, Ryan de M Jennings, Mark A Kozubal, Susannah G Tringe, Michael Wagner, William P Inskeep
Abstrakt

The candidate archaeal phylum 'Aigarchaeota' contains microorganisms from terrestrial and subsurface geothermal ecosystems. The phylogeny and metabolic potential of Aigarchaeota has been deduced from several recent single-cell amplified genomes; however, a detailed description of their metabolic potential and in situ transcriptional activity is absent. Here, we report a comprehensive metatranscriptome-based reconstruction of the in situ metabolism of Aigarchaeota in an oxic, hot spring filamentous 'streamer' community. Fluorescence in situ hybridization showed that these newly discovered Aigarchaeota are filamentous, which is consistent with the presence and transcription of an actin-encoding gene. Aigarchaeota filaments are intricately associated with other community members, which include both bacteria (for example, filamentous Thermocrinis spp.) and archaea. Metabolic reconstruction of genomic and metatranscriptomic data suggests that this aigarchaeon is an aerobic, chemoorganoheterotroph with autotrophic potential. A heme copper oxidase complex was identified in the environmental genome assembly and highly transcribed in situ. Potential electron donors include acetate, fatty acids, amino acids, sugars and aromatic compounds, which may originate from extracellular polymeric substances produced by other microorganisms shown to exist in close proximity and/or autochthonous dissolved organic carbon (OC). Transcripts related to genes specific to each of these potential electron donors were identified, indicating that this aigarchaeon likely utilizes several OC substrates. Characterized members of this lineage cannot synthesize heme, and other cofactors and vitamins de novo, which suggests auxotrophy. We propose the name Candidatus 'Calditenuis aerorheumensis' for this aigarchaeon, which describes its filamentous morphology and its primary electron acceptor, oxygen.The ISME Journal advance online publication, 3 July 2015; doi:10.1038/ismej.2015.83.

Organisation(en)
Externe Organisation(en)
United States Department of Energy, Montana State University, Bozeman, Indiana University Bloomington, Pacific Northwest National Laboratory, Sustainable Bioproducts LLC
Journal
The ISME Journal: multidisciplinary journal of microbial ecology
Band
10
Seiten
210–224
Anzahl der Seiten
15
ISSN
1751-7362
DOI
https://doi.org/10.1038/ismej.2015.83
Publikationsdatum
01-2016
Peer-reviewed
Ja
ÖFOS 2012
106022 Mikrobiologie
ASJC Scopus Sachgebiete
Ecology, Evolution, Behavior and Systematics, Microbiology
Sustainable Development Goals
SDG 15 – Leben an Land
Link zum Portal
https://ucrisportal.univie.ac.at/de/publications/0b1231dd-9590-47ed-bc7e-74dc21c98cab