Sequestration of translation initiation factors in p62 condensates

Autor(en)

Alberto Danieli, Georg Vucak, Manuela Baccarini, Sascha Martens

Abstrakt

Selective autophagy mediates the removal of harmful material from the cytoplasm. This cargo material is selected by cargo receptors, which orchestrate its sequestration within double-membrane autophagosomes and subsequent lysosomal degradation. The cargo receptor p62/SQSTM1 is present in cytoplasmic condensates, and a fraction of them are constantly delivered into lysosomes. However, the molecular composition of the p62 condensates is incompletely understood. To obtain insights into their composition, we develop a method to isolate these condensates and find that p62 condensates are enriched in components of the translation machinery. Furthermore, p62 interacts with translation initiation factors, and eukaryotic initiation factor 2α (eIF2α) and eIF4E are degraded by autophagy in a p62-dependent manner. Thus, p62-mediated autophagy may in part be linked to down-regulation of translation initiation. The p62 condensate isolation protocol developed here may facilitate the study of their contribution to cellular quality control and their roles in health and disease.

Organisation(en)

Department für Biochemie und Zellbiologie, Max Perutz Labs, Department für Mikrobiologie, Immunbiologie und Genetik

Journal

Cell Reports

Band

42

ISSN

2211-1247

DOI

https://doi.org/10.1016/j.celrep.2023.113583

Publikationsdatum

12-2023

Peer-reviewed

Ja

ÖFOS 2012

106023 Molekularbiologie, 106002 Biochemie

Schlagwörter

ASJC Scopus Sachgebiete

Allgemeine Biochemie, Genetik und Molekularbiologie, Biochemistry

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/4373ed5c-48b3-4226-803c-1d06c5d1ef5a