Unified Simulation Platform for Interference Microscopy

Autor(en)

Felix Hitzelhammer, Anežka Dostálová, Ilia Zykov, Barbara Platzer, Clara Conrad-Billroth, Thomas Juffmann, Ulrich Hohenester

Abstrakt

Interferometric scattering microscopy is a powerful technique that enables various applications, such as mass photometry and particle tracking. Here, we present a numerical toolbox to simulate images obtained in interferometric scattering, coherent bright-field, and dark-field microscopies. The scattered fields are calculated using a boundary element method, facilitating the simulation of arbitrary sample geometries and substrate layer structures. A fully vectorial model is used for simulating the imaging setup. We demonstrate excellent agreement between our simulations and experiments for different shapes of scatterers and excitation angles. Notably, for angles near the Brewster angle, we observe a contrast enhancement which may be beneficial for nanosensing applications. The software is available as a matlab toolbox.

Organisation(en)

Quantenoptik, Quantennanophysik und Quanteninformation, Department für Strukturbiologie und Computational Biology

Externe Organisation(en)

Karl-Franzens-Universität Graz, Universität Wien, Max F. Perutz Laboratories GmbH (MFPL), Olmützer Palacky-Universität

Journal

ACS Photonics

Band

11

Seiten

2745-2756

Anzahl der Seiten

12

ISSN

2330-4022

DOI

https://doi.org/10.1021/acsphotonics.4c00621

Publikationsdatum

07-2024

Peer-reviewed

Ja

ÖFOS 2012

103045 Lichtoptische Mikroskopie

Schlagwörter

ASJC Scopus Sachgebiete

Electronic, Optical and Magnetic Materials, Biotechnology, Atomic and Molecular Physics, and Optics, Electrical and Electronic Engineering

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/32e20438-c9a7-4d01-adee-60e73d1a4f2a