Ultraviolet irradiation induces autofluorescence enhancement via production of reactive oxygen species and photodecomposition in erythrocytes

Autor(en)

Xian Wu, Leiting Pan, Zhen-Hua Wang, Xiaoli Liu, Dan Zhao, Xin-Zheng Zhang, Romano Rupp, Jing-Jun Xu

Abstrakt

Ultraviolet (UV) light has a significant influence on human health. In this study, human erythrocytes were exposed to UV light to investigate the effects of UV irradiation (UVI) on autofluorescence. Our results showed that high-dose continuous UVI enhanced erythrocyte autofluorescence, whereas low-dose pulsed UVI alone did not have this effect. Further, we found that H2O2, one type of reactive oxygen species (ROS), accelerated autofluorescence enhancement under both continuous and pulsed UVI. In contrast, continuous and pulsed visible light did not result in erythrocyte autofluorescence enhancement in the presence or absence of H2O2. Moreover, NAD(P)H had little effect on UVI-induced autofluorescence enhancement. From these studies, we conclude that UVI-induced erythrocyte autofluorescence enhancement via both UVI-dependent ROS production and photodecomposition. Finally, we present a theoretical study of this autofluorescence enhancement using a rate equation model. Notably, the results of this theoretical simulation agree well with the experimental data further supporting our conclusion that UVI plays two roles in the autofluorescence enhancement process.

Organisation(en)

Physik Funktioneller Materialien

Externe Organisation(en)

Nankai University

Journal

Biochemical and Biophysical Research Communications

Band

396

Seiten

999-1005

Anzahl der Seiten

7

ISSN

0006-291X

DOI

https://doi.org/10.1016/j.bbrc.2010.05.044

Publikationsdatum

2010

Peer-reviewed

Ja

ÖFOS 2012

1030 Physik, Astronomie

Sustainable Development Goals

SDG 3 – Gesundheit und Wohlergehen

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/2e6af86e-4320-472c-baf3-92c45fc43711