Tutorial for the growth and development of Myxococcus xanthus as a Model System at the Intersection of Biology and Physics

Autor(en)

Jesus Manuel Antúnez Domínguez, Laura Pérez García, Natsuko Rivera-Yoshida, Jasmin Di Franco, David Steiner, Alejandro V. Arzola, Mariana Benítez, Charlotte Hamngren Blomqvist, Roberto Cerbino, Caroline Beck Adiels, Giovanni Volpe

Abstrakt

Myxococcus xanthus is a unicellular organism whose cells possess the ability to move and communicate, leading to the emergence of complex collective properties and behaviours. This has made it an ideal model system to study the emergence of collective behaviours in interdisciplinary research efforts lying at the intersection of biology and physics, especially in the growing field of active matter research. Often, challenges arise when setting up reliable and reproducible culturing protocols. This tutorial provides a clear and comprehensive guide on the culture, growth, development, and experimental sample preparation of \textit{M. xanthus}. Additionally, it includes some representative examples of experiments that can be conducted using these samples, namely motility assays, fruiting body formation, predation, and elasticotaxis.

Organisation(en)

Computergestützte Physik und Physik der Weichen Materie

Externe Organisation(en)

University of Gothenburg, Universidad Nacional Autonoma de Mexico

Publikationsdatum

07-2024

ÖFOS 2012

103015 Kondensierte Materie, 103045 Lichtoptische Mikroskopie, 106006 Biophysik

Schlagwörter

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/269b0719-3bfe-45bc-b980-a6d5a297cffc