Ultraviolet Irradiation-Dependent Fluorescence Enhancement of Hemoglobin Catalyzed by Reactive Oxygen Species

Autor(en)

Leiting Pan, Xiaoxu Wang, Shuying Yang, Xian Wu, Imshik Lee, Xin-Zheng Zhang, Romano Rupp, Jing-Jun Xu

Abstrakt

Ultraviolet (UV) light has a potent effect on biological organisms. Hemoglobin, an oxygen-transport protein, plays an irreplaceable role in sustaining life of all vertebrates. In this study we scrutinize the effects of ultraviolet irradiation (UVI) as well as visible irradiation on the flourescence characteristics of bovine hemoglobin (BHb) in vitro. Data show that UVI results in flourescence enhancement of BHb in a dose-dependent manner. Furthermore, UVI-induced flourencence enhancement is significantly increased when BHb is pretreated with hydrogen peroxide (H2O2), a tye of reactive oxygen species (ROS). Meanwhile, The water-soluble antioxidant vitamin C suppresses this UVI-induced flourescence enhancement. In contrast, green light irradiation does not lead to flourescence enhancement of BHb no matter whether H2O2 is acting on the BHb solution or not. Taken together, these results indicate that catalysis of ROS and UVI-dependent irradiation play two key roles in the process of UVI-induced flourescence enhancement of BHb.

Organisation(en)

Physik Funktioneller Materialien

Externe Organisation(en)

Nankai University

Journal

PLoS ONE

Band

7

Anzahl der Seiten

6

ISSN

1932-6203

DOI

https://doi.org/10.1371/journal.pone.0044142

Publikationsdatum

2012

Peer-reviewed

Ja

ÖFOS 2012

103018 Materialphysik

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/20309e9a-c40b-41b7-9c93-95ad3a6d1b1a