Effects of storage on stability and performance of carboxybetaine-based polymer brushes

Autor(en)

Markéta Vrabcová, Milan Houska, Monika Spasovová, Michala Forinová, Alina Pilipenco, Ivana Matoušová Víšová, Kateřina Mrkvová, Hana Vaisocherová-Lísalová

Abstrakt

Zwitterionic poly(carboxybetaine) brushes have emerged as promising surface functional coatings for antifouling label-free biosensing in complex biological fluids. This study investigates the effect of storage conditions on highly-dense poly(carboxybetaine methacrylamide) (pCBMAA), poly(sulfobetaine methacrylamide) (pSBMAA), a random copolymer of CBMAA with poly(hydroxypropyl methacrylamide) p(CBMAA-HPMAA), and terpolymer p(CBMAA-SBMAA-HPMAA) brushes. Synthesized via surface-initiated atom transfer radical polymerization (SI ATRP) on gold-coated substrates, these brushes were stored in dry, water, or phosphate-buffered saline (PBS) environments at temperatures of 22 °C, 6 °C, or frozen at –20 °C for 44 days. Structural stability of the brushes was assessed using infrared reflection-absorption spectroscopy (IRRAS) and spectroscopic ellipsometry (SE), while fouling resistance and IgG antibody binding capacity were evaluated using surface plasmon resonance (SPR). Although the brushes exhibited minimal detachment from the surface, a slight decrease in swelling ratio was observed across all storage conditions. Furthermore, the storage led to a minor reduction in IgG binding capacity, with freezing at –20 °C showing a more pronounced effect in most cases. Notably, storing all tested carboxybetaine-based brushes in water or in PBS at 6 °C adversely affected fouling, a phenomenon alleviated when stored frozen at –20 °C or in a dry state.

Organisation(en)

Department für Strukturbiologie und Computational Biology, Quantenoptik, Quantennanophysik und Quanteninformation

Externe Organisation(en)

Czech Academy of Sciences, Charles University Prague

DOI

https://doi.org/10.1117/12.3017613

Publikationsdatum

06-2024

Peer-reviewed

Ja

ÖFOS 2012

103021 Optik, 103023 Polymerphysik

Schlagwörter

ASJC Scopus Sachgebiete

Electronic, Optical and Magnetic Materials, Condensed Matter Physics, Computer Science Applications, Applied Mathematics, Electrical and Electronic Engineering

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/137475fa-18e2-4dad-884b-e117e30c66c2